Biotin-PEG3-Methyl ethanethioate Of NHS ester PEG Is For Targeted Drug Delivery

Important Product Information

? Biotin-PEG3-Methyl ethanethioate is moisture-sensitive. Store the vial of biotin reagent at 4°C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.

? As directed in the procedure, dissolve the NHS ester reagent immediately before use. The NHS moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time, and do not prepare stock solutions for storage. Discard any unused reconstituted reagent.

? Avoid buffers containing primary amines (e.g., Tris or glycine) as these will compete with the reaction. If necessary, dialyze or desalt to exchange the protein sample into an amine-free buffer such as phosphate-buffered saline (PBS).

? When Pegylating proteins in solution, excess non-reacted PEG linker is easily removed by size exclusion using either desalting columns or dialysis (Additional Information). A 10mL desalting column is best suited for processing Pegylation reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller amounts of protein and/or smaller reaction volumes, both the Pegylation reaction and subsequent buffer exchange may be performed in a single Thermo Scientific Slide-A-Lyzer MINI Dialysis Unit. For larger reaction volumes than can be processed with a desalting column, either split the sample between two columns.