t-Boc-N-amido-PEG24-acid (CAS: 2839118-68-0) is an atypical bifunctional, orthogonally protected PEG linker. Its structure consists of three parts: one end features a tertiary butyloxycarbonyl (t-Boc)-protected amine, providing chemical inertness; the middle section is a flexible PEG chain composed of 24 ethylene glycol units, enhancing water solubility and biocompatibility; the other end is an active carboxyl group (-COOH), which can undergo amidation reactions with amines. The production process employs a modular synthesis strategy, ensuring molecular uniformity through precise control over the introduction of the t-Boc protecting group and extension of the PEG chain. This reagent is widely used in the development of antibody-drug conjugates (ADCs), site-specific modification of proteins/polypeptides, and functionalization of nanomaterials, serving as a key tool for precision medicine and biotechnology.